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a Neonatal
Intensive Care Unit, Fazakerley Hospital, Liverpool, b Regional Public Health Laboratory, Fazakerley
Hospital, Liverpool
Correspondence to: Mr G Jawaheer, Department of Paediatric Surgery, Royal Hospital for Sick Children, Edinburgh EH9 1LF.
Accepted 5 November 1996
A prospective, blind study was carried out to determine: the
amount of blood submitted for culture from neonates; whether small
blood volumes resulted in false negative results; and whether there was
a temporal relation between volume of blood cultured and time to positivity.
Seventy three bottles were evaluated. They contained a median
of 0.63 ml of blood. Twenty nine bottles (39.7%) contained less than
0.5 ml of blood; 21 bottles (28.8%) were positive. There were three
false negative cultures, only one of which contained a blood volume
below 0.5 ml. The median time to positivity was 22.4 hours. There was
no correlation between blood volume cultured and time to positivity.
Neonatal cultures frequently contain less than 0.5 ml of
blood. False negative cultures are rare. Neonatal blood culture bottles
need to be validated for blood volumes below 0.5 ml.
This article has been cited by other articles:
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  H. W. Kilbride, R. Powers, D. D. Wirtschafter, M. B. Sheehan, D. S. Charsha, M. LaCorte, N. Finer, and D. A. Goldmann Evaluation and Development of Potentially Better Practices to Prevent Neonatal Nosocomial Bacteremia Pediatrics, April 1, 2003; 111(4): e504 - 518. [Abstract] [Full Text] [PDF]
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