Reduced expression of C5a receptors on neutrophils from cord blood
a Department of Clinical Biochemistry, Odense
University Hospital, b Department of Medical Microbiology
Correspondence to: Dr M Nybo Department of Clinical Biochemistry Odense University Hospital DK-5000 Odense C Denmark.m.nybo{at}winsloew.ou.dk
Accepted 28 August 1997
AIM
To describe further functional deficiencies of
neonatal neutrophils by measuring the expression of C5a receptors.
METHODSC5a uptake was measured using flow
cytometry with fluorescein isothiocynate labelled recombinant C5a. The
response of neutrophils to stimulation with C5a and fMLP was tested by
measuring migration and exocytosis of myeloperoxidase and lactoferrin.
RESULTSC5a mean fluorescence on neutrophils from
neonates was significantly lower (22.4 (SD 3.5)) than in adult controls
(31.5 (3.1)). Neutrophils from neonates migrated poorly towards both
C5a and fMLP compared with those from adult controls. Exocytosis of
myeloperoxidase, but not lactoferrin from neonatal neutrophils
stimulated with C5a, was significantly lower than in adult controls.
fMLP stimulation, on the other hand, resulted in significantly higher
exocytosis in neonates.
CONCLUSIONThe lower expression of C5a receptors
on neutrophils from neonates could be related to reduced C5a mediated
exocytosis of myeloperoxidase.
© 1998 by Archives of Disease in Childhood
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