Cytomegalovirus DNA detection on Guthrie cards in patients with neonatal cholestasis
a Department of
Paediatrics Huddinge University Hospital
Karolinska Institute
S-141 86 Huddinge
Sweden, b Department of Immunology, Microbiology,
Pathology and Infectious Diseases, Division of Clinical Virology
Correspondence to: Dr B Fischler.
Accepted 19 September
1998
AIM
To time the onset
of cytomegalovirus (CMV) infection in patients (n=39) with CMV
associated neonatal cholestasis by analysing CMV DNA on Guthrie cards
sampled at 3 days of age.
METHODSCMV infection
was diagnosed by serology/urine isolation or by CMV DNA detection
(polymerase chain reaction) in liver biopsy specimens. In order to time
the infection dry blood filter paper discs were punched out from stored
Guthrie cards. After phenol-chloroform extraction CMV DNA was detected
by nested polymerase chain reaction.
RESULTSAll cards from
control children (n=8) with congenital CMV tested positive; none of the
negative controls (n=4) did so. Two of 39 cholestatic infants were CMV
DNA positive; their mothers had serological signs compatible with
infection during the second half of the pregnancy. All other
cholestatic infants tested negative.
CONCLUSIONSCMV DNA
was not detected in most of the children using Guthrie cards,
suggesting that infection developed at or soon after birth.
© 1999 by Archives of Disease in Childhood
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