Developmental changes in erythrocyte Na+,K+-ATPase subunit abundance and enzyme activity in neonates
Barna Vasarhelyia, Tivadar Tulassayb, Agota Verc, Mariann Dobosb, Istvan Kocsisb, Istvan Serid
a Joint Research
Program of the Hungarian Academy of Sciences and the 1st Department of
Pediatrics, Semmelweis University School of Medicine, Budapest, Hungary, b 1st Department of
Pediatrics, Semmelweis University School of Medicine, Budapest, Hungary, c Department of Medical Chemistry, Molecular
Biology and Pathophysiology, Semmelweis University School of Medicine, d Division
of Neonatology, Department of Pediatrics, The Children's Hospital of
Philadelphia and the University of Pennsylvania, Philadelphia, PA, USA
Correspondence to: Dr Seri, The Children's Hospital of Philadelphia, University of Pennsylvania, 34th Street and Civic Center Blvd, Philadelphia, PA 19104, USA email: Seri{at}emailchop.edu
Accepted 28 April 2000
AIM
To study the
relation between erythrocyte Na+,K+-ATPase
subunit isoform composition, Na+,K+-ATPase
activity, and cation pump function in preterm and term neonates.
DESIGNErythrocyte
Na+,K+-ATPase subunit isoform
abundance, Na+,K+-ATPase activity, and cation
pump function were studied in blood samples obtained from 56 preterm
neonates of 28-32 weeks gestation (group 1), 58 preterm neonates of
33-36 weeks gestation (group 2), and 122 term neonates (group 3)
during the first two postnatal days.
RESULTS
1
isoform abundance was higher and 
2 isoform abundance was
lower in group 1 than in group 3 (p = 0.0002). 2 and
1 isoform abundance did not change with maturation and
there was no evidence for the presence of the 3 isoform.
Gestational age was inversely related to
Na+,K+-ATPase activity (p = 0.0001) and
directly related to intracellular Na+ concentration
(p = 0.0025).
CONCLUSIONSExpression
of the 1 and 2
Na+,K+-ATPase subunit isoforms is
developmentally regulated. The increased abundance of 1
isoforms of immature neonates translates to increased ATPase activity. The lower intracellular Na+ concentration of immature
neonates suggests that their erythrocyte Na+,K+-ATPase cation pump function may also be increased.
Keywords: Na+,K+-ATPase activity; subunit isoforms; sodium concentration; preterm; erythrocyte; cation pump
© 2000 by Archives of Disease in Childhood
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