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Arch Dis Child Fetal Neonatal Ed 2000;83:F135-F138 ( September )

Developmental changes in erythrocyte Na+,K+-ATPase subunit abundance and enzyme activity in neonates

Barna Vasarhelyia, Tivadar Tulassayb, Agota Verc, Mariann Dobosb, Istvan Kocsisb, Istvan Serid

a Joint Research Program of the Hungarian Academy of Sciences and the 1st Department of Pediatrics, Semmelweis University School of Medicine, Budapest, Hungary, b 1st Department of Pediatrics, Semmelweis University School of Medicine, Budapest, Hungary, c Department of Medical Chemistry, Molecular Biology and Pathophysiology, Semmelweis University School of Medicine, d Division of Neonatology, Department of Pediatrics, The Children's Hospital of Philadelphia and the University of Pennsylvania, Philadelphia, PA, USA

Correspondence to: Dr Seri, The Children's Hospital of Philadelphia, University of Pennsylvania, 34th Street and Civic Center Blvd, Philadelphia, PA 19104, USA email: Seri{at}emailchop.edu

Accepted 28 April 2000

AIM---To study the relation between erythrocyte Na+,K+-ATPase subunit isoform composition, Na+,K+-ATPase activity, and cation pump function in preterm and term neonates.
DESIGN---Erythrocyte Na+,K+-ATPase subunit isoform abundance, Na+,K+-ATPase activity, and cation pump function were studied in blood samples obtained from 56 preterm neonates of 28-32 weeks gestation (group 1), 58 preterm neonates of 33-36 weeks gestation (group 2), and 122 term neonates (group 3) during the first two postnatal days.
RESULTS---alpha 1 isoform abundance was higher and beta 2 isoform abundance was lower in group 1 than in group 3 (p = 0.0002). alpha 2 and beta 1 isoform abundance did not change with maturation and there was no evidence for the presence of the alpha 3 isoform. Gestational age was inversely related to Na+,K+-ATPase activity (p = 0.0001) and directly related to intracellular Na+ concentration (p = 0.0025).
CONCLUSIONS---Expression of the alpha 1 and beta 2 Na+,K+-ATPase subunit isoforms is developmentally regulated. The increased abundance of alpha 1 isoforms of immature neonates translates to increased ATPase activity. The lower intracellular Na+ concentration of immature neonates suggests that their erythrocyte Na+,K+-ATPase cation pump function may also be increased.


Keywords: Na+,K+-ATPase activity; subunit isoforms; sodium concentration; preterm; erythrocyte; cation pump

© 2000 by Archives of Disease in Childhood


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Na pump isoforms in human erythroid progenitor cells and mature erythrocytes
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[Abstract] [Full Text] [PDF]


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