Type I collagenases in bronchoalveolar lavage fluid from preterm babies at risk of developing chronic lung disease

doi:10.1136/fn.84.3.F168

Type I collagenases in bronchoalveolar lavage fluid from preterm babies at risk of developing chronic lung disease

D G Sweet^a, K J McMahon^b, A E Curley^a, C M O'Connor^b, H L Halliday^a

^a Department of Child Health, The Queen's University of Belfast and Regional Neonatal Unit, Royal Maternity Hospital, Belfast, Northern Ireland, ^b Department of Medicine and Therapeutics, University College Dublin, Republic of Ireland

Correspondence to: Dr Sweet, Perinatal Room, Royal Maternity Hospital, Grosvenor Road, Belfast BT12 6BB, Northern Ireland dsweet{at}dnet.co.uk

Accepted 23 November 2000

OBJECTIVE $---$ To assess whether increased collagenolysis precedes severe chronic lung disease(CLD).
METHODS $---$ Matrix metalloproteinase-1 (MMP-1) and MMP-8 (enzymes that degrade type I collagen, the main structural protein of lung extracellularmatrix) were measured by enzyme linked immunosorbent assay in100 bronchoalveolar lavage samples taken during the first 6 postnataldays from 45 ventilated preterm babies < 33 weeks gestation. Themedian value for each baby was calculated. CLD was defined asan oxygen requirement after the 36th week afterconception.
RESULTS $---$ MMP-8 levels in bronchoalveolar lavage fluid were higher (median 13 ng/ml) in 20 babies who developed CLD than in 25 withoutCLD (median 2 ng/ml). No MMP-1 was detected in anysample.
CONCLUSIONS $---$ MMP-8 can be detected in bronchoalveolar lavage fluid from preterm babies, and higher levels are found in those who laterdevelop CLD. MMP-8 may contribute to lung injury that occurs asa prelude toCLD.

Keywords: chronic lung disease; lungs; collagen; extracellular matrix; matrix metalloproteinase; preterm

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