Cytomegalovirus DNA detection on Guthrie cards in patients with neonatal cholestasis
- aDepartment of Paediatrics Huddinge University Hospital Karolinska Institute S-141 86 Huddinge Sweden, bDepartment of Immunology, Microbiology, Pathology and Infectious Diseases, Division of Clinical Virology
- Dr B Fischler.
- Accepted 19 September 1998
Abstract
AIM To time the onset of cytomegalovirus (CMV) infection in patients (n=39) with CMV associated neonatal cholestasis by analysing CMV DNA on Guthrie cards sampled at 3 days of age.
METHODS CMV infection was diagnosed by serology/urine isolation or by CMV DNA detection (polymerase chain reaction) in liver biopsy specimens. In order to time the infection dry blood filter paper discs were punched out from stored Guthrie cards. After phenol–chloroform extraction CMV DNA was detected by nested polymerase chain reaction.
RESULTS All cards from control children (n=8) with congenital CMV tested positive; none of the negative controls (n=4) did so. Two of 39 cholestatic infants were CMV DNA positive; their mothers had serological signs compatible with infection during the second half of the pregnancy. All other cholestatic infants tested negative.
CONCLUSIONS CMV DNA was not detected in most of the children using Guthrie cards, suggesting that infection developed at or soon after birth.
