Table 3
CMV primers used for PCR A, B, and C
| Viral region CMV MIE | Localisation | Sequences | Length base pairs |
|---|---|---|---|
| OP1 | −22 to −2 | 5′-GCAG AGCT CGTT TAGT GAAC C-3′ | |
| OP2 | 66-85 | 5′-CCGT TCCC GGCC GCGG AGGC-3′ | 107 |
| IEP2A | 1479-1498 | 5′-ATGG AGTC CTCT GCCA AGAG-3′ | |
| IEP4B | 2182-2201 | 5′-CAAT ACAC TTCA TCTC CTCG-3′ | 723 |
| IEP3A | 1677-1696 | 5′-GTGA CCAA GGCC ACGA CGTT-3′ | |
| IEP3B | 1824-1843 | 5′-TCTG CCAG GACA TCTT TCTC-3′ | 167 |
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MIE = major immediate early; OP = oligonucleotide primer; IEP = immediate early primer.
