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Arch Dis Child Fetal Neonatal Ed 80:F226-F229 doi:10.1136/fn.80.3.F226
  • Original article

Correction for erythroid cell contamination in microassay for immunophenotyping of neonatal lymphocytes

Table 2

Illustration of erythroid cell contamination bias in two cord blood samples2-150

Gestational age Erythroid cells within “lympho-gate” CD3+T lymphocytes CD19+B lymphocytes CD3CD16/56+natural killer cells Lympho-sum
Normoblasts Unlysed Uncorrected2-151 Corrected2-152 Uncorrected2-151 Corrected2-152 Uncorrected2-151 Corrected2-152 Uncorrected2-151 Corrected2-152
29 weeks 44 5 28 54 18 36 10 20 56 110
35 weeks 40 3 24 43 10 17 17 30 51 89
  • 2-150 All numbers represent percentages.

  • 2-151 Without correction for the erythroid contamination of the lympho-gate.

  • 2-152 With correction for the erythroid contamination of the lympho-gate.

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