Type I collagenases in bronchoalveolar lavage fluid from preterm babies at risk of developing chronic lung disease
- aDepartment of Child Health, The Queen's University of Belfast and Regional Neonatal Unit, Royal Maternity Hospital, Belfast, Northern Ireland, bDepartment of Medicine and Therapeutics, University College Dublin, Republic of Ireland
- Dr Sweet, Perinatal Room, Royal Maternity Hospital, Grosvenor Road, Belfast BT12 6BB, Northern Irelanddsweet{at}dnet.co.uk
- Accepted 23 November 2000
Abstract
OBJECTIVE To assess whether increased collagenolysis precedes severe chronic lung disease (CLD).
METHODS Matrix metalloproteinase-1 (MMP-1) and MMP-8 (enzymes that degrade type I collagen, the main structural protein of lung extracellular matrix) were measured by enzyme linked immunosorbent assay in 100 bronchoalveolar lavage samples taken during the first 6 postnatal days from 45 ventilated preterm babies < 33 weeks gestation. The median value for each baby was calculated. CLD was defined as an oxygen requirement after the 36th week after conception.
RESULTS MMP-8 levels in bronchoalveolar lavage fluid were higher (median 13 ng/ml) in 20 babies who developed CLD than in 25 without CLD (median 2 ng/ml). No MMP-1 was detected in any sample.
CONCLUSIONS MMP-8 can be detected in bronchoalveolar lavage fluid from preterm babies, and higher levels are found in those who later develop CLD. MMP-8 may contribute to lung injury that occurs as a prelude to CLD.
